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Mitosis Manipulatives Kit
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Mitosis Manipulatives Kit
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DNA Manipulatives Kit
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DNA Manipulatives Kit
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Protein Synthesis Manipulatives Kit
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Protein Synthesis Manipulatives Kit
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IBI PCR DNA Fragment Extraction Kits
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The Gel/PCR DNA Fragments Extraction Kits are designed to recover or concentrate DNA fragments (50bp- 10kb) from an agarose gel, PCR, or any other enzymatic reaction. This method uses a chaotropic salt, guanidine thiocyanante, to dissolve the agarose gel and denature the enzymes. The DNA fragments in the chaotropic salt are bound to the glass-fiber matrix of the spin column. After washing off the contaminants, the purified DNA fragments are eluted by a low salt elution buffer or water. Salts, enzymes, and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation.
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IBI Mini Hi-Speed Plasmid Kits
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The IBI High-Speed Plasmid Mini Kit is designed for the rapid isolation of plasmid or cosmid DNA from 1-4ml of bacterial cultures. The modified alkaline lysis method and RNase treatment are used to create cleared cell lysate with minimal genomic DNA and RNA contaminants. In the presence of a chaotropic salt, the plasmid DNA in the lysate, binds to the glass-fiber matrix in the spin column. The purified plasmid DNA is eluted by a low salt elution buffer or water. This procedure does not require DNA phenol extraction or alcohol precipitation. Typical yields are 20-30?g of DNA for high-copy number plasmid or 3-10?g for low-copy plasmid.
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IBI Midi/Maxi Fast Ion Plasmid Kits
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IBI’s Plasmid Midi/Maxi Kits use pre-packed ion-exchange resin columns to purify plasmid or cosmid DNA from bacterial cultures. In this process, the modified alkaline lysis method and RNase treatment are used for creating cleared cell lysate with minimal genomic DNA and RNA contaminants. Using a gravity-flow procedure, the plasmid DNA in crude lysate has been bound to the column. The contaminants can be washed off with wash buffer. Finally, the purified plasmid DNA is eluted by a high salt buffer and then precipitated with isopropanol for desalting. The entire procedure can be completed in less than 2 hours without ultracentrifuges or HPLC and other hazardous reagents.
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IBI 96-Well Plasmid Kits
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The 96-Well Plasmid Kit is designed for high-throughput plasmid or cosmid DNA from 1-2ml of bacterial cultures. The modified alkaline lysis method and centrifugation are used for creating cleared cell lysate with minimal genomic DNA and RNA contaminants. Binding, washing, and elution steps can be done using a vacuum manifold or by centrifugation.
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IBI Mini Genomic DNA Kits (Blood)
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The Genomic DNA Kit (Blood and Cultured Cell) is specially designed for purification of total DNA (including: genomic, mitochondrial, and viral DNA) from different volumes of blood samples and cultured cells. In this procedure, RBC Lysis Buffer is used to remove non-nucleated red blood cells and reduce hemoglobin contamination.
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IBI Mini Genomic DNA Kits (Tissue)
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The Genomic DNA Mini Kit (Tissue) is specially designed for purification of total DNA (including: genomic, mitochondrial, and viral DNA) from a variety of animal tissues or cells. Provided micropestle can efficiently homogenize tissue samples to shorten the time on the lysis step. This method uses proteinase K and chaotropic salt to lyse cells and degrade protein, then DNA in chaotropic salt is bound to the glass fiber matrix of the column. After washing off the contaminants, the purified DNA is eluted by a low salt elution buffer or water.
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IBI Mini Genomic DNA Kits (Plant)
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The Genomic DNA Mini/Maxi Kits (Plant) provide a fast and simple method to isolate total DNA (genomic DNA, mitochondrial, chloroplast and viral DNA) from plant tissue and cells. Plant tissues are ground in liquid nitrogen and lysed by a buffer containing detergent. The tissue debris in lysate can be removed by the provided filter column. In the presence of a chaotropic salt, the genomic DNA in the lysate binds to the glass fiber matrix in the spin column. After washing off the contaminants, the purified DNA is eluted by a low salt elution buffer or water.
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IBI Mini Total RNA Kits (Tissue)
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The Total RNA Mini Kit (Tissue) is specially designed for purification of Total RNA from a variety of animal tissues cocells. The provided micropestle can efficiently homogenize tissue samples in the microcentrifuge tube. This method uses detergents and a chaotropic salt to lyse cell and inactivate RNase, then RNA in the chaotropic salt is bonded to the glassfiber matrix of the column. After washing off the contaminants, the purified RNA is eluted by RNase-free water.
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IBI Mini Total RNA Kits (Blood)
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The Total RNA Mini Kit (Blood and Cultured Cells) is specially designed for purification of total RNA from cultured cells and fresh whole human blood. This method uses detergents and a chaotropic salt to lyse cells and inactivate RNase then, RNA in chaotropic salt is bonded to the glass-fiber matrix of the column. After washing off the contaminants, the purified RNA is eluted by RNase-free water.
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IBI Mini Total RNA Kits (Plant)
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The Total RNA Mini Kit (Plant) provides a fast and simple method to isolate total RNA from plant tissue and cells. In this process, the sample is distributed by grinding it in liquid nitrogen and filtrated by using a filter column to remove cell debris. In the presence of binding buffer with chaotropic salt, the total RNA in the lysate binds to the glass-fiber matrix in the spin column. The optional DNase treatments are used to remove DNA residues, and the contaminants are washed with an ethanol based wash buffer. Finally, the purified total RNA is eluted by RNase-free water. The protocol does not require phenol extraction or alcohol precipitation. The entire procedure can be completed in 60 minutes, and the purified Total RNA is ready for RT, RT-PCR, Real-Time PCR, or Northern Blotting.
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PCR cleanup UNIFILTER®
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Process 96 or 384 samples quickly with greater than 85% recovery. The Whatman PCR Cleanup UNIFILTER eliminates time-consuming precipitations and labour-intensive resin purifications……
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UNIPLT 384WELL 100U C-PS 50/PK (Product Code: QF)
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List Price: $130.50
BLD Price $117.45
You Save $13.05!
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UNIPLT 96WL 250UL V PP-50/pk (Product Code: QF)
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List Price: $162.90
BLD Price $159.61
You Save $3.29!
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UN 96 800u PSC GF/F PHI LGDRIP 25/EA (Product Code: QF)
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List Price: $467.00
BLD Price $457.57
You Save $9.43!
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384 DNA BINDING PLATE-50/Pk (Product Code: QF)
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List Price: $1,060.90
BLD Price $954.81
You Save $106.09!
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